(D) Quantitation of transcript degradation ( + + ).įunctional Splice Sites Increase the Stability of Pol II Transcripts (C) Quantitation of the data in (B) by computing the fraction spliced ( + )/ ( + + ). The pre-synthesized pre-mRNA (right) is capped. Pre-mRNA transcript, spliced product, and intermediate lariat bands are indicated. For the transcription/splicing UTP chase experiment, the zero time point refers to the initiation of the chase reaction through the addition of excess unlabeled UTP. (B) Representative autoradiograms show the time course of the transcription/splicing UTP chase experiment for Pol II- (left) or T7- (middle) generated transcripts, and the splicing profile of pre-synthesized pre-mRNAs (right). (A) Diagrams of the wt β-globin minigene DNA template with the Pol II promoter (top), the T7 promoter (middle), or the pre-synthesized RNA transcript (lower). The Link between Pol II Transcription and Splicing Increases the Stability of Nascent Transcripts but Not the Rate of Splicing Molar concentration of RNA was determined by normalizing counts per min to a known concentration of radiolabeled RNA. Pre-mRNA transcripts were either synthesized by Pol II (left) or T7 polymerase (right). (B) Representative autoradiograms of the in vitro transcription/splicing assay appear below the diagrams. ![]() The template on the left is headed by the AdML Pol II promoter while the right template is headed by the T7 promoter. (A) Diagrams of the two templates used in the transcription/splicing assay, both contain the first exons of the β-globin gene. Utilize the massive potential of transcription-related content and make it available to customers online.Linking Splicing to Pol II Transcription Increases the Efficiency of Pre-mRNA Splicing Thus, reduce the workload and keep up with the times by using the power of transcription services. In other words, just one video can be used as a reference for many things, which is why it's advantageous to transcribe the video, creating an additional reference. The video may be less than ten minutes, yet contain a wealth of information that can be expanded upon through blogs, newsletters and social media. One of the ways to maximize web content is to create multiple uses for the same video. Transcribing a marketing meeting video helps give customers deeper insight and access to unique product information that may not be available elsewhere online. Sometimes consumers want to research this information quickly and don't have time to watch videos, so transcriptions can help cut to the chase.īy recording some of your marketing meetings and making them available to customers online, you can earn their trust that you are by sharing what seems to be inside information. Videos of customers giving reviews of your product can be very influential in helping other consumers decide if they want to try your product. If you make a sales video that gives details about a product, the transcription can show up in search engines to give you an edge over competitors that do not offer videos or comprehensive product descriptions. The more content you have indexed in search engines, the more chances you have to be found by people interested in your niche. One of the most valuable aspects of transcribing your audio or video files is that it can add content to your website and feed search engines with deeper information than just basic tags and descriptions. ![]() The following details about transcription services will help provide your customers with the content they are looking for. This strategy can be very useful if you make demonstration videos or promotional videos that need to be transcribed. Instead of a business spending time digitizing all their documents, the work can be outsourced to transcription services. As businesses go more digital, there is a growing demand for digital documents online that consumers can access.
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